Have you ever been infected with new coronavirus, do you still need to be vaccinated?

New England Journal of Medicine   Recently, we published a report on the effectiveness of bnt162b2 mRNA vaccine in patients with previous infection

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The researchers found that vaccination elicited a stronger immune response in people with a history of new coronavirus infection than in people who had never been infected with new coronavirus

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The clinical study included 188 healthy subjects, 36 of whom were 30-60 days after new coronavirus infection, and 152 had no history of new coronavirus infection

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Using Merck milliplex ® The IgG antibody levels of spike protein subunits S1, S2, RBD and N of NCV were detected by multiple factor detection kit

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After the first dose of vaccine, the levels of anti-s1 and anti-s2 antibodies in the two groups increased, while the level of anti-s1 and anti-s2 antibodies in the N protein did not increase because it was not a vaccine antigen

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Compared with those who had never been infected with NCV, those who had recently been infected had significantly higher levels of RBD neutralizing antibody 3 weeks after the first dose of vaccine (96-97% vs 49.6-66.2%)

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The current research results suggest that the immunity of the vaccine to the new coronavirus may not last long, and the antibody will decline over time

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So people who have been infected with the new coronavirus still need to be vaccinated

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A report on cell suggests that cytokine storms may prevent infected people from developing long-term immunity 2

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The researchers observed the lymph nodes and spleen of patients with novel coronavirus death, and found that the germinal center structure has not yet formed, which is the necessary basis for the formation of a lasting immune response

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Cytokine storm is no stranger

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We have realized that novel coronavirus infection can induce significant changes in a variety of cytokines and chemokines

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A report from nature uses Merck milliplex ® The changes of 23 cytokines and chemokines in rhesus monkeys infected with NCV were comprehensively monitored by multiple factor detection technology

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On the first day after infection, IL-1ra, IL-6, IL-10, IL-15, MCP-1 and MIP-1 were detected β On the third day after infection, TGF – β increased significantly α The level of blood glucose decreased significantly

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Although some of the cytokines changed at subsequent time points, these changes were not significant

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Among them, some high levels of cytokines seem to be associated with persistent immunity

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In severe patients with novel coronavirus, the release of TNF was the highest

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The researchers explored the effect of TNF on persistent immunity in mouse models and found that TNF seemed to prevent the formation of germinal centers in mice infected with neocoronavirus

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When mice were injected with antibodies to prevent TNF or deleted TNF gene, germinal centers could be formed

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When the researchers studied the lymph nodes of patients with novel coronavirus death, they found high levels of TNF in these organs

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Therefore, they concluded that TNF may prevent the formation of germinal centers in patients with novel coronaviruss

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Whether viral infection or vaccine immunization, they are very complex physiological processes, involving a variety of signal molecules, such as antibodies, cytokines and so on

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With the spread of the epidemic, the genome of the new coronavirus is constantly mutating

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Therefore, there is still a long way to go for the development of new vaccine

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Whether in the R & D stage of animal model construction or in the later clinical stage, comprehensive monitoring of these signal molecules is the basis for our objective evaluation of vaccine effect

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The new coronavirus kit can detect IgA, IgM or IgG antibodies, and each kit can simultaneously detect S1, S2, RBD and N proteins

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The user may need to select all or part of the analytes

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Each kit contains all the reagents and 96 well plates needed for testing

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Each kit contains a set of positive quality control and a negative quality control for the performance test of the kit

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The intra plate CV was controlled within 15% (8 reportable results), and the inter plate CV was within 20% (across 4 different assessments)

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In order to let more researchers know milliplex ®、 Better use of milliplex ®, Merck launched milliplex ®  Bimonthly magazine, updated regularly, using milliplex ®  Published top-level articles covering covid-19, cancer, neurology, cardiovascular and other fields

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